亞細胞定位預測的方法——Cell-PLoc 2.0
這是一款快速預測蛋白質的亞細胞定位預測方法的網站,網站的預測方法發表在Nature protocols 上,網站地址:
http://www。
csbio。sjtu。edu。cn/bioin
f/Cell-PLoc-2/
Reference: Kuo-Chen Chou, and Hong-Bin Shen, Cell-PLoc 2。0: an improved package of web-servers for predicting subcellular localization of proteins in various organisms, Natural Science, 2010, 2: 1090-1103
1。進入網址之後,我們看到如下網頁介面
2。我們選擇真核蛋白作為一個例子進行預測。
3。我們輸入一個真核蛋白,以Fasta格式,我們就以網站給出的Example序列為例
NOTE: The FASTA format for the current predictor can be described as follows。
(1) The first line of each query protein input format must begin with a greater-than (“>”) symbol in the first column。 The word following the “>” symbol is the identifier and description of the sequence, but both are optional。
(2) The sequence (in single-character code) begins in a different line and ends if another line starting with a “>” appears, which indicates the start of another query protein。
4。結果輸出
可見示例蛋白為胞質表達,很簡單有木有?